RNA-seq and Differential Expression
Overview
Instructor: Wesley Brashear
Time: Friday, March 22, 2024 10:00AM-12:30PM CT
Location: Blocker 220
Prerequisites: Current HPRC account
This short course covers concepts of RNA-seq data analysis for differential expression, including Quality Control, reference-based read alignment, utilization of DESeq2, and data visualization in R.
Course Materials
- RNA-seq and Differential Expression (Spring 2024): PDF
Previous Semester Materials
- RNA-seq and Differential Expression (Fall 2022): PDF
Agenda
This course focuses, among others, on the following topics:
- Generating RNA-seq libraries
- Experimental design
- Transcriptome assembly overview
- Quality Control/Library trimming
- Aligning RNA-seq libraries to a reference genome
- Generating count files
- Differential expression in R
- Small RNA analysis