RNA-seq and Differential Expression
Overview
Instructor: Wesley Brashear
Time: Friday, October 18, 2024 — 10:00AM-12:30PM CT
Location: Blocker 220
Prerequisites: Active HPRC account
This short course covers concepts of RNA-seq data analysis for differential expression, including Quality Control, reference-based read alignment, utilization of DESeq2, and data visualization in R.
Course Materials
- RNA-seq and Differential Expression (Fall 2024): PDF
Previous Semester Materials
Learning Objectives and Agenda
In this course, participants will:
- Learn technical considerations that need to be addressed when designing an RNA-seq experiment
- Learn how to conduct quality control assessments of RNA-seq libraries
- Learn how to align RNA-seq libraries to a reference genome
- Learn how to conduct differential expression analysis and data visualization in R
This course focuses, among others, on the following topics:
- RNA-seq library preparation methods
- Experimental design
- Transcriptome assembly overview
- Quality Control/Library trimming
- Aligning RNA-seq libraries to a reference genome
- Generating count files
- Differential expression in R